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Uploader: Konstantina Diamantara
Contributor: Anja Zeigerer, Jerome Gilleron, Konstantina Diamantara, Marino Zerial
Other contributors: Not specified
Version: 1
Projects: A3.2: Cross-talk of signaling pathways and endocytic machinery in hepato..., C: Liver lobule level
Scales: Liver lobule and Liver
Created: 6th Dec 2013 at 10:37 Last updated: 11th Jan 2016 at 15:18
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Download Uploader: Konstantina Diamantara
Contributor: Anja Zeigerer, Jerome Gilleron, Konstantina Diamantara, Marino Zerial
Other contributors: Not specified
Version: 1
Projects: A3.2: Cross-talk of signaling pathways and endocytic machinery in hepato...
Scales: Intercellular and Liver lobule
Created: 6th Dec 2013 at 10:36 Last updated: 11th Jan 2016 at 15:18
Uploader: Konstantina Diamantara
Contributor: Anja Zeigerer, Hidenori Nonaka, Konstantina Diamantara, Marino Zerial
Other contributors: Not specified
Version: 1
Projects: A3.2: Cross-talk of signaling pathways and endocytic machinery in hepato..., A3.3: Hepatocyte polarity, A3.5: The impact of cell polarity on metabolism detoxification and endoc..., B3: Intercellular communication through cell adhesion
Scales: Cell, Intercellular and Liver
Created: 28th Feb 2014 at 12:34 Last updated: 11th Jan 2016 at 15:17
Uploader: Maria Thomas
Contributor: Maria Thomas, Ulrich Zanger
Other contributors: Not specified
Version: 1
Projects: A3.4: Linking signalling to metabolic functions, B5: Cell-cell communication influences detoxifying functions in hepatocytes, Showcase LPS and Inflammation, Showcase Steatosis, Virtual Liver
Scales: Cell
Chromatin immunoprecipitation (ChIP) is a valuable tool to detect the interaction between a transcription factor (TF) and DNA fragments in vivo. This technology enables distinguishing between direct and indirect responsive targets when combined with approaches of gene expression assessment following activation of a TF. ChIP may be used to confirm in vivo association of a transcriptional regulator with suspected target DNA fragments. Moreover, this method may also be used to discover new targets,
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Created: 24th Jan 2014 at 15:34 Last updated: 26th Jan 2014 at 07:50
Uploader: Maria Thomas
Contributor: Maria Thomas
Other contributors: Not specified
Version: 1
Projects: A3.4: Linking signalling to metabolic functions, B5: Cell-cell communication influences detoxifying functions in hepatocytes
Scales: Not specified
Here we used VSV-G-pseudotyped, EGFP-expressing lentiviral vectors to develop an efficient gene transfer protocol to modify gene expression in primary human hepatocytes (by RNAi). The protocol comprises the production of recombinant viruses as well as the steps for efficient delivery of short-hairpin RNA (shRNAs), microRNAs or anti-microRNAs to human hepatocytes. On average infection efficiencies of over 95% are achieved at relatively low multiplicity of infection (MOI), which effectively reduces
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Created: 4th Apr 2011 at 17:06 Last updated: 21st Jan 2013 at 12:16
Uploader: Bruno Christ
Contributor: Bruno Christ
Other contributors: Peggy Stock, Sabine Ebensing, Madlen Hempel, Matthias M Dollinger
Version: 1
Projects: C6: Organization and function of the sinusoidal system and the liver lob...
Scales: Liver lobule
This protocol describes the in vitro differentiation of stem cells from human bone marrow and their transplantation into livers of immunodeficient mice.
Created: 24th Oct 2012 at 10:34
