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Establishment of a rat model: Associating liver partition with portal vein ligation for staged hepatectomy

Abstract:

BACKGROUND: We adapted the anatomically oriented parenchyma-preserving resection technique for associating liver partition with portal vein ligation (PVL) for staged hepatectomy (ALPPS) in rats and examined the role of revascularization in intrahepatic size regulation.

METHODS: We performed the procedures based on anatomic study. The ALPPS procedure consisted of a 70% PVL (occluding the left median, left lateral, and right lobes), parenchymal transection (median lobe) and partial (10%) hepatectomy (PHx; caudate lobe). The transection effect was evaluated by measuring the extent of hepatic atrophy or regeneration of individual liver lobes in the ALPPS and control groups (70% PVL and 10% PHx without transection). The survival rates after stage II resection and collateral formation within the portal vein system was examined.

RESULTS: Anatomic study revealed a close spatial relationship between the demarcation line and the middle median hepatic vein. This enabled placing the transection plane without injuring the hepatic vein. Transection was achieved via stepwise clamping, followed by 2-3 parenchyma-preserving piercing sutures on both sides of the clamp. Ligated liver lobes atrophy was significantly enhanced after ALPPS compared with the control group. In contrast, both a significantly greater relative weight of the regenerated lobe and proliferation index on the first postoperative day were observed. All animals tolerated stage II-resection without complications. Portoportal collaterals were only observed in the control group.

CONCLUSION: We developed an anatomically precise technique for parenchymal transection. The lack of a dense vascular network between the portalized and deportalized lobes may play an important role in accelerating regeneration and atrophy augmentation.

10.1016/j.surg.2015.12.005

Projects: D4: Regeneration and Liver Size, E3: Horizontal integration on organ scale

Surgery
Surgery 159(5) : 1299
1st May 2016

Weiwei Wei, Tianjiao Zhang, Sara Zafarnia, Andrea Schenk, Chichi Xie, Chunyi Kan, Olaf Dirsch, Utz Settmacher, Uta Dahmen

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[Weiwei Wei] [Sara Zafarnia] [Andrea Schenk] [Olaf Dirsch] [Uta Dahmen]

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Liver
Views: 660
  • Created: 26th May 2017 at 10:46
  • Last updated: 26th May 2017 at 10:47

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