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A simple TLC-MALDI method to monitor oxidation products of phosphatidylcholines and -ethanolamines

Abstract:

The analysis of lipid/phospholipid oxidation products is of primary importance. Although there are established HPLC and LC/MS techniques, it is shown here for the first time that the combination of matrix-assisted laser desorption and ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) and TLC represents a sensitive, fast and convenient alternative. A mixture of 1-palmitoyl-2-linoleoyl-sn-phosphatidylcholine (PLPC) and -ethanolamine (PLPE) was oxidized under the influence of atmospheric oxygen and characterized by direct positive ion MALDI-TOF MS as well as combined TLC/MALDI. It is shown that much more detailed information – particularly related to the oxidation products of PLPE that have so far been scarcely investigated – can be obtained by TLC/MALDI. However, it is also shown that further methodological improvements are necessary to make this method generally applicable to complex lipid mixtures.

10.1556/AChrom.23.2011.2.13

Projects: A1.1: Central liver metabolism and its regulation under nutritional chal...

Acta Chromatographica
Acta Chromatographica 23(2) : 365
1st Jun 2011

A. Bischoff, M. Eibisch, B. Fuchs, R. Süss, M. Schürenberg, D. Suckau, J. Schiller

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[Mandy Eibisch] [Beate Fuchs] [Jürgen Schiller]

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  • Created: 23rd Jun 2011 at 17:24
  • Last updated: 24th Oct 2013 at 16:18

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