A simple TLC-MALDI method to monitor oxidation products of phosphatidylcholines and -ethanolamines
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The analysis of lipid/phospholipid oxidation products is of primary importance. Although there are established HPLC and LC/MS techniques, it is shown here for the first time that the combination of matrix-assisted laser desorption and ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) and TLC represents a sensitive, fast and convenient alternative. A mixture of 1-palmitoyl-2-linoleoyl-sn-phosphatidylcholine (PLPC) and -ethanolamine (PLPE) was oxidized under the influence of atmospheric oxygen and characterized by direct positive ion MALDI-TOF MS as well as combined TLC/MALDI. It is shown that much more detailed information – particularly related to the oxidation products of PLPE that have so far been scarcely investigated – can be obtained by TLC/MALDI. However, it is also shown that further methodological improvements are necessary to make this method generally applicable to complex lipid mixtures.
Projects: A1.1: Central liver metabolism and its regulation under nutritional chal...
Acta Chromatographica
Acta Chromatographica 23(2) : 365
1st Jun 2011
A. Bischoff, M. Eibisch, B. Fuchs, R. Süss, M. Schürenberg, D. Suckau, J. Schiller
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- Created: 23rd Jun 2011 at 17:24
- Last updated: 24th Oct 2013 at 16:18
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