A simple TLC-MALDI method to monitor oxidation products of phosphatidylcholines and -ethanolamines


The analysis of lipid/phospholipid oxidation products is of primary importance. Although there are established HPLC and LC/MS techniques, it is shown here for the first time that the combination of matrix-assisted laser desorption and ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) and TLC represents a sensitive, fast and convenient alternative. A mixture of 1-palmitoyl-2-linoleoyl-sn-phosphatidylcholine (PLPC) and -ethanolamine (PLPE) was oxidized under the influence of atmospheric oxygen and characterized by direct positive ion MALDI-TOF MS as well as combined TLC/MALDI. It is shown that much more detailed information – particularly related to the oxidation products of PLPE that have so far been scarcely investigated – can be obtained by TLC/MALDI. However, it is also shown that further methodological improvements are necessary to make this method generally applicable to complex lipid mixtures.


Projects: A1.1: Central liver metabolism and its regulation under nutritional chal...

Acta Chromatographica
Acta Chromatographica 23(2) : 365
1st Jun 2011

A. Bischoff, M. Eibisch, B. Fuchs, R. Süss, M. Schürenberg, D. Suckau, J. Schiller

help Authors

[Mandy Eibisch] [Beate Fuchs] [Jürgen Schiller]

help Attributions


help Scales

Not Specified
Views: 2449
  • Created: 23rd Jun 2011 at 17:24
  • Last updated: 24th Oct 2013 at 16:18

Related items


Log in / Register

Need an account?
Sign up

Forgotten password?

Front Page

Virtual Liver Network


Related Projects and friends

Imprint Taverna workflow workbench myExperiment JWS Online ISATAB myGrid Sabio-RK BioPortal Semantic SBML

Powered by:


Silk icons 1.3
Crystal Clear icons