Phosphatidylcholine dimers can be easily misinterpreted as cardiolipins in complex lipid mixtures: a matrix-assisted laser desorption/ionization time-of-flight mass spectrometric study of lipids from hepatocytes


The liver is an important organ that is particularly involved in the lipid metabolism of the organism. Thus, high interest is nowadays focused on the lipid composition of the liver and particularly the liver parenchymal cells, the hepatocytes. Hepatocytes contain common phospholipids (PL) such as phosphatidylcholines, -ethanolamines and -inositols, for instance, that can be easily analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) even without previous separation of the PL mixture. However, in addition to common PL, hepatocytes possess also significant amounts of cardiolipin (CLP). The MS analysis of this PL is quite challenging because it (a) has a higher mass than common lipids and (b) possesses a higher negative charge. We will show here that caution is required if CLP is analyzed directly from the total lipid extract because PC dimers may be interpreted as cardiolipins if the positive ion MALDI mass spectra are analyzed.


Projects: A1.1: Central liver metabolism and its regulation under nutritional chal...

Rapid Commun. Mass Spectrom.
Rapid Commun. Mass Spectrom. 25(18): 2619-26
30th Sep 2011

Mandy Eibisch, Sebastian Zellmer, Rolf Gebhardt, Rosmarie Süss, Beate Fuchs, Jürgen Schiller

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[Mandy Eibisch] [Sebastian Zellmer] [Rolf Gebhardt] [Beate Fuchs] [Jürgen Schiller]

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Cell and Liver
Views: 1775
  • Created: 8th Jan 2014 at 13:25

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