Wordprocessing

Computational processing and error reduction strategies for standardized quantitative data in biological networks

Abstract:

High-quality quantitative data generated under standardized conditions is critical for understanding dynamic cellular processes. We report strategies for error reduction, and algorithms for automated data processing and for establishing the widely used techniques of immunoprecipitation and immunoblotting as highly precise methods for the quantification of protein levels and modifications. To determine the stoichiometry of cellular components and to ensure comparability of experiments, relative signals are converted to absolute values. A major source for errors in blotting techniques are inhomogeneities of the gel and the transfer procedure leading to correlated errors. These correlations are prevented by randomized gel loading, which significantly reduces standard deviations. Further error reduction is achieved by using housekeeping proteins as normalizers or by adding purified proteins in immunoprecipitations as calibrators in combination with criteria-based normalization. Additionally, we developed a computational tool for automated normalization, validation and integration of data derived from multiple immunoblots. In this way, large sets of quantitative data for dynamic pathway modeling can be generated, enabling the identification of systems properties and the prediction of targets for efficient intervention.

16336276

Projects: HepatoSys

FEBS J.
FEBS J. 272(24): 6400-11
13th Dec 2005

Marcel Schilling, Thomas Maiwald, Sebastian Bohl, Markus Kollmann, Clemens Kreutz, Jens Timmer, Ursula Klingmüller

help Authors

[Thomas Maiwald] [Clemens Kreutz] [Jens Timmer] [Ursula Klingmüller]

help Attributions

None

help Scales


Not Specified
Views: 1665
  • Created: 23rd Aug 2012 at 13:22
  • Last updated: 24th Oct 2013 at 16:24

Related items

Ajax-loader-large

Log in / Register

Need an account?
Sign up

Forgotten password?

Front Page

Virtual Liver Network

(v.0.22.0)

Related Projects and friends


Imprint Taverna workflow workbench myExperiment JWS Online ISATAB myGrid Sabio-RK BioPortal Semantic SBML

Powered by:

Ror-logo-32

Icons:
Silk icons 1.3
Crystal Clear icons