Wordprocessing

Lipid analysis by thin-layer chromatography—A review of the current state

Abstract:

High-performance thin-layer chromatography (HPTLC) is a widely used, fast and relatively inexpensive method of separating complex mixtures. It is particularly useful for smaller, apolar compounds and offers some advantages over HPLC. This review gives an overview about the special features as well as the problems that have to be considered upon the HPTLC analysis of lipids. The term “lipids” is used here in a broad sense and comprises fatty acids and their derivatives as well as substances related biosynthetically or functionally to these compounds. After a short introduction regarding the stationary phases and the methods how lipids can be visualized on an HPTLC plate, the individual lipid classes will be discussed and the most suitable solvent systems for their separation indicated. The focus will be on lipids that are most abundant in biological systems, i.e. cholesterol and its derivates, glycerides, sphingo- and glycolipids as
well as phospholipids. Finally, a nowadays very important topic, the combination between HPTLC and mass spectrometric (MS) detection methods will be discussed. It will be shown that this is a very powerful method to investigate the identities of the HPTLC spots in more detail than by the use of common staining methods. Future aspects of HPTLC in the lipid field will be also discussed.

10.1016/j.chroma.2010.11.066

Projects: No Projects

Journal of Chromatography A
Journal of Chromatography A 1218(19) : 2754
1st May 2011

Beate Fuchs, Rosmarie Süß, Kristin Teuber, Mandy Eibisch, Jürgen Schiller

help Authors

[Beate Fuchs] [Mandy Eibisch] [Jürgen Schiller]

help Attributions

None

help Scales


Not Specified
Views: 1569
  • Created: 4th Aug 2012 at 16:19
  • Last updated: 24th Oct 2013 at 16:18

Related items

Ajax-loader-large

Log in / Register

Need an account?
Sign up

Forgotten password?

Front Page

Virtual Liver Network

(v.0.22.0)

Related Projects and friends


Imprint Taverna workflow workbench myExperiment JWS Online ISATAB myGrid Sabio-RK BioPortal Semantic SBML

Powered by:

Ror-logo-32

Icons:
Silk icons 1.3
Crystal Clear icons