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Response of hepatocytes to rapid change in the medium composition (gene regulatory network): Model 1100 freshMedia


Primary hepatocytes were exposed to a stimulus by exchanging culture medium, thereby simulating changes in the blood composition. The expression of genes at different time points was recorded. Differentially expressed genes were clustered using fuzzy c-means algorithm into five groups. The arcs of the possible network were identified using the NetGenerator algorithm under the restriction of biological knowledge. The analysis was restricted to the main metabolic pathways of hepatocytes. The reverse engineering approach was applied to validate the data and a network of interacting genes was generated. Pyruvate kinase and pyruvat carboxylase are genes that rapidly respond to the changes in the medium composition.

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Filename Format Size Action
FOSBE2007_FreshMedia.xml XML document 26.9 KB Download

Organism: Mus musculus

Model type: Not specified

Model format: SBML

Execution or visualisation environment: Not specified

Model image: (Click on the image to zoom)

help Contributors

[Data From HepatoSys] [Wolfgang Schmidt-Heck] [Sebastian Zellmer] [Rolf Gebhardt] [Reinhard Guthke]

help Attributions

None

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Views: 3388    Downloads: 1240
  • Created: 25th Jul 2011 at 14:48
  • Last updated: 24th Feb 2012 at 15:11
  • Last used: 24th Aug 2019 at 21:08

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