Response of hepatocytes to rapid change in the medium composition (gene regulatory network): Model 1100 freshMedia

Primary hepatocytes were exposed to a stimulus by exchanging culture medium, thereby simulating changes in the blood composition. The expression of genes at different time points was recorded. Differentially expressed genes were clustered using fuzzy c-means algorithm into five groups. The arcs of the possible network were identified using the NetGenerator algorithm under the restriction of biological knowledge. The analysis was restricted to the main metabolic pathways of hepatocytes. The reverse engineering approach was applied to validate the data and a network of interacting genes was generated. Pyruvate kinase and pyruvat carboxylase are genes that rapidly respond to the changes in the medium composition.

1 item (and an image) are associated with this Model: view in large icon

Filename Format Size Action
FOSBE2007_FreshMedia.xml XML document 26.9 KB Download

Organism: Mus musculus

Model type: Not specified

Model format: SBML

Execution or visualisation environment: Not specified

Model image: (Click on the image to zoom)

help Contributors

[Data From HepatoSys] [Wolfgang Schmidt-Heck] [Sebastian Zellmer] [Rolf Gebhardt] [Reinhard Guthke]

help Attributions


help Scales

Views: 3648    Downloads: 1275
  • Created: 25th Jul 2011 at 14:48
  • Last updated: 24th Feb 2012 at 15:11
  • Last used: 26th Oct 2020 at 11:35

Related items


Log in / Register

Need an account?
Sign up

Forgotten password?

Front Page

Virtual Liver Network


Related Projects and friends

Imprint Taverna workflow workbench myExperiment JWS Online ISATAB myGrid Sabio-RK BioPortal Semantic SBML

Powered by:


Silk icons 1.3
Crystal Clear icons