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Dynamic network reconstruction identified unexpected gene expression in primary hepatocytes after exchange of culture medium (FOSBE 2007 Contributed Paper)

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Primary hepatocytes were exposed to a stimulus by exchanging culture medium, thereby simulating changes in the blood composition. The expression of genes at different time points was recorded. Differentially expressed genes were clustered using fuzzy c-means algorithm into five groups. The arcs of the possible network were identified using the NetGenerator algorithm under the restriction of biological knowledge. The analysis was restricted to the main metabolic pathways of hepatocytes. The reverse engineering approach was applied to validate the data and a network of interacting genes was generated. Pyruvate kinase and pyruvat carboxylase are genes that rapidly respond to the changes in the medium composition.

Filename: FOSBE2007 SchmidtHeck.pdf

Format: PDF document

Size: 145 KB

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[Data From HepatoSys] [Wolfgang Schmidt-Heck] [Sebastian Zellmer] [Rolf Gebhardt] [Reinhard Guthke]

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Views: 2485    Downloads: 1196
  • Created: 1st Aug 2011 at 11:49
  • Last updated: 1st Aug 2011 at 13:43
  • Last used: 17th Jun 2019 at 04:02

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