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Genericblue SOP- IF imaging of lipid nanoparticles (LNPs) in perfused liver - Marino Zerial-MPI-CBG


Uploader: Konstantina Diamantara

Contributor: Anja Zeigerer, Jerome Gilleron, Konstantina Diamantara, Marino Zerial

Other contributors: Not specified

Scales: Liver lobule and Liver

No description specified

Created: 6th Dec 2013 at 10:37   Last updated: 11th Jan 2016 at 15:18


 

Genericblue SOP- EM of lipid nanoparticles (LNPs) in vivo - Marino Zerial-MPI-CBG


Uploader: Konstantina Diamantara

Contributor: Anja Zeigerer, Jerome Gilleron, Konstantina Diamantara, Marino Zerial

Other contributors: Not specified

Scales: Intercellular and Liver lobule

No description specified

Created: 6th Dec 2013 at 10:36   Last updated: 11th Jan 2016 at 15:18


 

Genericblue SOP In vivo LNP injections-Marino Zerial MPI-CBG


Uploader: Konstantina Diamantara

Contributor: Anja Zeigerer, Hidenori Nonaka, Konstantina Diamantara, Marino Zerial

Other contributors: Not specified

Scales: Cell, Intercellular and Liver

No description specified

Created: 28th Feb 2014 at 12:34   Last updated: 11th Jan 2016 at 15:17


 

Pdf SOP-ChromatinIP - IKP- Uli Zanger Group-IKP Stuttgart


Uploader: Maria Thomas

Contributor: Maria Thomas, Ulrich Zanger

Other contributors: Not specified

Scales: Cell

Chromatin immunoprecipitation (ChIP) is a valuable tool to detect the interaction between a transcription factor (TF) and DNA fragments in vivo. This technology enables distinguishing between direct and indirect responsive targets when combined with approaches of gene expression assessment following activation of a TF. ChIP may be used to confirm in vivo association of a transcriptional regulator with suspected target DNA fragments. Moreover, this method may also be used to discover new targets,
...

Created: 24th Jan 2014 at 15:34   Last updated: 26th Jan 2014 at 07:50


 

Pdf Introduction of shRNAs, miRNAs or anti-microRNAs into primary human hepatocytes with lentivirus


Uploader: Maria Thomas

Contributor: Maria Thomas

Other contributors: Not specified

Scales: Not specified

Here we used VSV-G-pseudotyped, EGFP-expressing lentiviral vectors to develop an efficient gene transfer protocol to modify gene expression in primary human hepatocytes (by RNAi). The protocol comprises the production of recombinant viruses as well as the steps for efficient delivery of short-hairpin RNA (shRNAs), microRNAs or anti-microRNAs to human hepatocytes. On average infection efficiencies of over 95% are achieved at relatively low multiplicity of infection (MOI), which effectively reduces
...

Created: 4th Apr 2011 at 17:06   Last updated: 21st Jan 2013 at 12:16


 

Pdf The generation of hepatocytes from mesenchymal stem cells and engraftment into murine liver


Uploader: Bruno Christ

Contributor: Bruno Christ

Other contributors: Peggy Stock, Sabine Ebensing, Madlen Hempel, Matthias M Dollinger

Scales: Liver lobule

This protocol describes the in vitro differentiation of stem cells from human bone marrow and their transplantation into livers of immunodeficient mice.

Created: 24th Oct 2012 at 10:34



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