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Synchrotron infrared spectroscopic ellipsometry for characterization of biofunctional surfaces

Abstract:

The structural and thickness homogeneity of different biofunctional surfaces after coating an Au substrate was studied by infrared spectroscopic ellipsometry (IRSE). Monolayer coverage was achieved by washing the samples in aqueous solution. A selected cysteine-modified CHL peptide and an anti-glutathione S-transfer (GST) antibody produced a characteristic change in the IR ellipsometric spectra after peptide and antibody adsorption, confirming the biosensing ability of this technique. IR ellipsometric mapping at the synchrotron storage ring BESSY II in Berlin allowed laterally resolved characterization of the protein films. The IR ellipsometric tanΨ and Δ maps revealed that the protein films are inhomogeneous in structure and thickness after adsorption of the peptide and antibody. The absolute thickness of the protein films varied considerably by up to 3 to 7 nm. We also studied cytosine films with different thicknesses (d = 58 nm and 125 nm) and a guanine film (d = 84 nm). For the two cytosine films the synchrotron spectra revealed that the thicker film in particular was rather homogeneous in thickness but inhomogeneous in structure. For the guanine film the shape of vibrational bands in the ellipsometric spectra correlated with anisotropic molecular orientations.

10.1002/pssb.200983945

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phys. stat. sol. (b)
phys. stat. sol. (b) 247(8) : 1925
1st Aug 2010

Guoguang Sun, Dana Maria Rosu, Xin Zhang, Marc Hovestädt, Simona Pop, Ulrich Schade, Dennis Aulich, Michael Gensch, Bernhard Ay, Hermann-Georg Holzhütter, Dietrich R. T. Zahn, Norbert Esser, Rudolf Volkmer, Jörg Rappich, Karsten Hinrichs

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[Hermann-Georg Holzhütter]

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Views: 1361
  • Created: 4th Jan 2013 at 12:51
  • Last updated: 24th Oct 2013 at 16:20

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