Response of sinusoidal mouse liver cells to choline-deficient ethionine-supplemented diet


Proliferation of oval cells, the bipotent precursor cells of the liver, requires impeded proliferation and loss of hepatocytes as well as a specific micro-environment, provided by adjacent sinusoidal cells of liver. Despite their immense importance for triggering the oval cell response, cells of hepatic sinusoids are rarely investigated. To elucidate the response of sinusoidal liver cells we have employed a choline-deficient, ethionine-supplemented (CDE) diet, a common method for inducing an oval cell response in rodent liver. We have utilised selected expression markers commonly used in the past for phenotypic discrimination of oval cells and sinusoidal cells: cytokeratin, E-cadherin and M2-pyruvate kinase for oval cells; and glial fibrillary acidic protein (GFAP) was used for hepatic stellate cells (HSCs).


Projects: A: Cellular level, B: Communication of Hepatocytes and Non-Parenchymal Liver Cells, C: Liver lobule level

Comp Hepatol
Comp Hepatol 9: 8
13th Oct 2010

Elke Ueberham, Jan Böttger, Uwe Ueberham, Jens Grosche, Rolf Gebhardt

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[Jan Böttger] [Rolf Gebhardt]

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Views: 2163
  • Created: 20th Jul 2012 at 10:16
  • Last updated: 24th Oct 2013 at 16:19

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