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Response of hepatocytes to rapid change in the medium composition (gene regulatory network)

Primary hepatocytes were exposed to a stimulus by exchanging culture medium, thereby simulating changes in the blood composition. The expression of genes at different time points was recorded. Differentially expressed genes were clustered using fuzzy c-means algorithm into five groups. The arcs of the possible network were identified using the NetGenerator algorithm under the restriction of biological knowledge. The analysis was restricted to the main metabolic pathways of hepatocytes. The reverse engineering approach was applied to validate the data and a network of interacting genes was generated. Pyruvate kinase and pyruvat carboxylase are genes that rapidly respond to the changes in the medium composition.

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Modelling Analysis

Projects: HepatoSys

Investigation: Exchanging culture medium

Study: Response of hepatocytes to rapid change in the medium composition

Biological problem addressed: Gene expression

Organisms:
Mus musculus : C57BL/6N (wild-type / wild-type) : [Hepatocyte] (primary cell culture)

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[Data From HepatoSys]

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Cell
Views: 1864
  • Created: 1st Aug 2011 at 15:44
  • Last updated: 24th Jul 2015 at 11:50

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