Assays

 

Flask3-64x64 EM analysis of LNP-gold-labelled-siRNAs trafficking in vivo


ID: 77

Assay type: Rnai

Technology type: Technology type

Investigation: 1 hidden item

Study: Image-based analysis of lipid nanoparticle-medi...

Contributor: Konstantina Diamantara

Projects: A3.2: Cross-talk of signaling pathways and endocytic machinery in hepato...

Organisms: Not Specified

SOPs: SOP- EM of lipid nanoparticles (LNPs) in vivo -...

Data files: No Data files

Scales: Intercellular and Liver lobule

No description specified

Created: 6th Dec 2013 at 12:00   Last updated: 11th Jan 2016 at 15:18


 

Flask3-64x64 In vivo LNP injections


ID: 100

Assay type: Rnai

Technology type: Technology type

Investigation: 1 hidden item

Study: In vivo Rab5 knock down

Contributor: Konstantina Diamantara

Projects: A3.2: Cross-talk of signaling pathways and endocytic machinery in hepato...

Organisms: Not Specified

SOPs: SOP In vivo LNP injections-Marino Zerial MPI-CBG

Data files: No Data files

Scales: Cell, Intercellular and Liver

No description specified

Created: 28th Feb 2014 at 12:36   Last updated: 11th Jan 2016 at 15:18


 

Flask3-64x64 In vitro knock down of Rab5


ID: 99

Assay type: Rnai

Technology type: Technology type

Investigation: 1 hidden item

Study: In vitro knock down of Rab5

Scales: Cell and Liver

No description specified

Created: 28th Feb 2014 at 11:11   Last updated: 11th Jan 2016 at 15:18


 

Flask3-64x64 in vivo fluorescence analysis of LNP-siRNA-Alexa647


ID: 78

Assay type: Rnai

Technology type: fluorescence

Investigation: 1 hidden item

Study: Image-based analysis of lipid nanoparticle-medi...

Scales: Liver lobule and Liver

No description specified

Created: 6th Dec 2013 at 12:06   Last updated: 11th Jan 2016 at 15:18


 

Desktop Kinetic modelling of hepatic glucose metabolism


Contributor: Matthias König

Projects: A1.1: Central liver metabolism and its regulation under nutritional chal..., A3.4: Linking signalling to metabolic functions

Organisms: Not Specified

SOPs: No SOPs

Data files: No Data files

Scales: Cell and Liver

No description specified

Created: 3rd Nov 2013 at 16:43   Last updated: 24th Jul 2015 at 13:31


 

Desktop From Hepatocytes to Whole Liver Function: A Multi-Scale Model of Human Hepatic Galactose Metabolism


ID: 71

Biological problem addressed: Metabolic network

Investigation: Investigation: Multi-scale model galactose meta...

Study: Study : Galactose metabolism in the liver

Models: 2 hidden items

Scales: Cell, Liver lobule and Liver

We present a multi-scale model of liver galactose metabolism bridging the scales from single-cell metabolism over the tissue level to the whole-organ. The model combines a detailed kinetic model of the cellular galactose metabolism with a tissue-scale perfusion model of the sinusoid. The metabolic capacity of the whole liver is modelled by integrating the heterogeneous contribution of sinusoids differing in blood-flow rates and tissue-architecture. The model was applied to the normal physiological
...

Created: 3rd Nov 2013 at 15:17   Last updated: 24th Jul 2015 at 13:30


 

Desktop GRN2SBML: encoding of gene regulatory network models


ID: 70

Biological problem addressed: Gene regulatory network

Investigation: 1 hidden item

Study: 1 hidden item

Models: 4 hidden items

Contributor: Sebastian Vlaic

Projects: A: Cellular level

Organisms: Not Specified

SOPs: No SOPs

Data files: GRN2SBML Bundle

Scales: Cell

No description specified

Created: 1st Nov 2013 at 10:42   Last updated: 24th Jul 2015 at 13:29


 

Flask3-64x64 Fluidigm dynamic arrays for TaqMan® PCR


ID: 32

Assay type: Transcriptional profiling

Technology type: fluidigm high-throughput taqman platform

Investigation: 1 hidden item

Study: 1 hidden item

Contributor: Maria Thomas

Projects: A3.4: Linking signalling to metabolic functions, Showcase Steatosis

Organisms:
Homo sapiens : [Hepatocyte] (primary cell culture)

SOPs: 2 hidden items

Data files: 5 hidden items

Scales: Not specified

A technology which offers on-chip networks of microfluidc channels.This platform enables us to perform high-throughput real-time PCR with up to 9.216 data points in one run in TaqMan data quality.

Created: 6th Nov 2012 at 06:47   Last updated: 24th Jul 2015 at 12:18


 

Desktop Response of hepatocytes to rapid change in the medium composition (gene regulatory network)


Contributor: Data From HepatoSys

Projects: HepatoSys

Organisms:
Mus musculus : C57BL/6N (wild-type / wild-type) : [Hepatocyte] (primary cell culture)

SOPs: No SOPs

Data files: Dynamic network reconstruction identified unexp...

Scales: Cell

Primary hepatocytes were exposed to a stimulus by exchanging culture medium, thereby simulating changes in the blood composition. The expression of genes at different time points was recorded. Differentially expressed genes were clustered using fuzzy c-means algorithm into five groups. The arcs of the possible network were identified using the NetGenerator algorithm under the restriction of biological knowledge. The analysis was restricted to the main metabolic pathways of hepatocytes. The reverse
...

Created: 1st Aug 2011 at 15:44   Last updated: 24th Jul 2015 at 11:50


 

Flask3-64x64 Response of hepatocytes to rapid change in the medium composition (gene regulatory network)


Contributor: Data From HepatoSys

Projects: HepatoSys

Organisms:
Mus musculus : C57BL/6N (wild-type / wild-type) : [Hepatocyte] (primary cell culture)

SOPs: No SOPs

Data files: Dynamic network reconstruction identified unexp...

Scales: Not specified

Primary hepatocytes were exposed to a stimulus by exchanging culture medium, thereby simulating changes in the blood composition. The expression of genes at different time points was recorded. Differentially expressed genes were clustered using fuzzy c-means algorithm into five groups. The arcs of the possible network were identified using the NetGenerator algorithm under the restriction of biological knowledge. The analysis was restricted to the main metabolic pathways of hepatocytes. The reverse
...

Created: 1st Aug 2011 at 15:27   Last updated: 24th Jul 2015 at 11:48



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